Journal: Frontiers in Physiology

Article Title: Thiamet-G facilitates reparative dentin formation via modulating O-GlcNAcylation and inflammation

doi: 10.3389/fphys.2025.1739168

Figure Lengend Snippet: Effect of Thiamet-G on human dental pulp stem cells (hDPSCs). (A) Experimental design for MTS assay to examine cell viability and proliferation. (B) MTS assay shows that Thiamet-G treatment facilitates dose-dependent hDPSC proliferation. (C) Experimental design for ALP activity assay. (D) Thiamet-G treatment significantly induces ALP activity in hDPSCs compared to the control, particularly after 14 days (E) Thiamet-G treatment increases the expression of reparative dentin-related markers, including Alp, Bmp2, Bsp, Dspp, Opn, Gsk3β, and RUNX2, compared to the control. Ns non-significant, * p < 0.03, ** p < 0.002, *** p < 0.0002, and **** p < 0.0001. NC negative control, GM growth medium, OM osteogenic medium.

Article Snippet: After 24 h, the media was changed with osteogenic media (Alfa-MEM with 1% penicillin-streptomycin, 5% fetal bovine serum, 50 μM L-ascorbic acid, 10 mM β-glycerophosphate, and 100 nM dexamethasone) in presence or absence of Thiamet-G (S7213, Selleckchem.com ) with various concentrations.

Techniques: MTS Assay, ALP Activity Assay, Activity Assay, Control, Expressing, Negative Control

Journal: Bone Reports

Article Title: Two children with hypophosphatasia with a heterozygous c.1559delT variant in the ALPL gene, the most common variant in Japanese populations

doi: 10.1016/j.bonr.2022.101626

Figure Lengend Snippet: Change in biomarkers related to HPP before treatment with asfotase alfa in Case 2.

Article Snippet: Laboratory findings were normal, except for a low serum ALP level (112 IU/L, measured using the International Federation Clinical Chemistry and Laboratory Medicine: IFCC method; reference interval, 161–508 IU/L), which was overlooked by the pediatrician.

Techniques: